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Hilo Core Genomics Facility (HCGF)
The core genomics facility functions as a support lab, containing instruments for various molecular biological uses, including:
- AB 3500 Genetic Analyzer
- CEQ 8000 Genetic Analysis Systems
- AB StepOne Plus Real-Time PCR
- electrophoresis systems
- NanoDrop spectrophotometer
- Revco freezers (for storage)
- Qubit 3.0 Fluorometer
- Agilent 2100 Bioanalyzer
- E-gel electrophoresis system
- Ion Torrent Next-generation Sequencing including the Ion Chef system
Reference materials and analytical software are also available. The facility provides DNA sequencing, genotyping services such as SNP,AFLP,STR, NGS, and Real-Time PCR. The HCGF runs workshops and training sessions to introduce current molecular and biotechnological techniques to students, faculty, and researchers as well as elementary and high school teachers. Current workshops include DNA isolation, PCR basics, gel electrophoresis, microsatellite basics, DNA sequencing, Real-Time PCR basics, and molecular cloning. The HCGF gives researchers, educators, and students the opportunity to gain access to technological services and and technical training. The HCGF is currently collaborating with the following institutions: UH-Hilo, UH-Manoa, Hawaii Community College, USGS-Biological Resource Division, USDA, Hawaii DLNR_ Division of Aquatic Resources, USNPS, Smithsonian Institute, UC-Berkeley, Humboldt State University, Auburn University, Stanford University, and Bishop Museum.
The laboratory’s mission is to support research at the University of Hawaii, with a primary focus on water quality and ecological research. The facility provides analytical services for researchers throughout the University of Hawaii system, as well as for investigators from federal, state, and country agencies in Hawaii and other universitieis. The services provided by the laboratory include:
- Nutrient analyses of water samples using a Lachat Quikchem 8500(Lachat Hach Company)
- Total organic carbon and total nitrogen analysis of water samples by high-temperature combustion using a TOC/TN Analyzer ( Shimadzu TOC-V, TNM-1)
- Particulate carbon and nitrogen analyses of soils, sediments, plant materials, plankton and filters using high temperature combustion ( Costech Elemental Analyzer)
- Stable isotopic analyses of particulate carbon and nitrogen for soils, plant materials, plankton, filters, and animal tissue samples using an Isotope Ratio Mass Spectrometer (Thermo-Finnegan Delta V IRMS)
- Elemental analysis of major and minor elements in water, plant materials, soils and sediments, and animal tissue using an ICP-OES Spectrometer (Varian Vista MPX)Nutrient analyses of KCl soil samples using an AutoAnalyzer (AAI, Pulse Instrumentation, Labtronics )
The Analytical Laboratory is utilized by: UH-Hilo TCBES, UH-Hilo Departments of Marine Science, Chemistry, Geology, Biology, Agriculture, and Aquaculture; UH-Manoa Departments of Oceanography, Geology, Botany, and College of Tropical Agriculture; USDA Forest Service; USGS- Biological Resources Discipline; National Park Service; State of Hawaii Department of Health; Stanford University; UC-Berkeley; Oregon State University and UCLA.
Spatial Data Analysis and Visualization Labs (SDAV)
This facility is a cyber-geo-eco informatics center that provides resources to faculty, graduate and undergraduate students, federal and state agencies and faculty from other universities. The facility provides access to state-of-the-art Geographic Information Systems (GIS), Remote Sensing (RS) and Global Positioning System (GPS) equipment. Field equipment available through SDAL includes:
- Differential GPS units
- Handheld GPS units
- Laser-locator binoculars
- Tablet PCs
Airborne digital imagery collectors and the Hawaii IKONOS Consortium (HIC) for satellite imagery are available through partnerships with other campuses and agencies. The facility provides training that empowers faculty and students to use visualization, modeling, and supercomputer facilities. SDAL provides easy access to Hawaii-based weather, climate, hydrologic, and soils data; mapping services including data collection, analysis, production, and training. Technical support in designing, implementing, and maintaining field sensor networks and associated communication systems is available. SDAK is currently used by faculty members at UH-Hilo and Hawaii Community College, staff members, and graduate and undergraduate student researchers.
Pacific Aquaculture and Coastal Resources Center (PACRC)
The Pacific Aquaculture and Coastal Resources Center is a research and education facility, with a mission in supporting research and education in coastal studies, aquaculture, and marine projects.
The Pacific Aquaculture & Coastal Resources Center's mission is to advance long-term sustainable use and conservation of coastal areas worldwide through aquaculture and resource management. The Center promotes excellence and innovation in interdisciplinary scientific research, public policy initiatives, outreach activities, and education.
The PACRC provides:
- research space for students and faculty
- filtered sea water for experiments, projects, and classes
- Salt, brackish, or freshwater aquaculture systems
- a water quality laboratory
- a pathology laboratory
- quarantine facilities
- a freshwater fish hatchery
- a operation oyster hatchery
Growth and holding facilities for aquatic projects are also available. Current projects include a student run oyster hatchery, studies on a variety of native Hawaiian fish, a Marine Science wave radar project, and water quality analyses of waters around Hilo, Hawaii. The Pacific Aquaculture & Coastal Resources Center has an array of ongoing projects worldwide. The Center is currently involved with aquaculture and coastal resource management work in the Marshall Islands, the Federated States of Micronesia, Nicaragua, and Tanzania. Current partners include: UH SeaGrant, Pacific Basin Agricultural Research Center, the Hawaii State Department of Health, Paepae o Heeia Fishpond, Kualoa Ranch, and UH Manoa.
Find us on Facebook: http://www.facebook.com/pacrc
Last updated: November 2016